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Neostigmine is a prescription drug that treats myasthenia gravis, urinary retention, and reverses neuromuscular blockade. Learn about its dosages, side effects, drug interactions, warnings, and precautions from RxList. Neostigmine Methylsulfate, a cholinesterase inhibitor, is indicated for reversal of the effects of nondepolarizing neuromuscular blocking agents (NMBA) after surgery (1). The drug information on NEOSTIGMINE include structure, IUPAC, physiochemical properties, synthesis, SAR, MOA, therapeutic uses, side effects and MCQs for GPAT and other exam preparations. Neostigmine is a water-soluble, ionized compound that reversibly inhibits acetylcholinesterase and is an FDA-approved agent for reversing non-depolarizing neuromuscular blockade after surgery.