Introduction to HPLC High Performance Liquid Chromatography ( HPLC ) is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent (mobile phase) flowing through a column packed with a separation medium (stationary phase). Sample components separate from one another by a process of differential migration as they flow through the column. What is HPLC (High-performance liquid chromatography )? High-Performance Liquid Chromatography ( HPLC ) is a sophisticated analytical technique used extensively in various scientific fields to separate, identify, and quantify the components of a mixture. This technique, originally known as high-pressure liquid chromatography , has evolved significantly since its inception. HPLC operates on the principle of column chromatography but utilizes advanced technology to enhance its performance. Unlike ... High-performance liquid chromatography ( HPLC ) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially … High-performance liquid chromatography ( HPLC ) is a powerful analytical technique used in chemistry, biochemistry, and pharmaceutical sciences for the separation, identification, and quantification of chemical compounds in complex mixtures.
