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Centrifugation is a laboratory technique that involves the separation of particles from a liquid based on their density and size differences using centrifugal force. Preparatory centrifugation deals with the isolation and purification of components such as tissue, cells, subcellular structure, membrane vesicles, and other particles of biochemical interest. In contrast, analytical centrifugation is carried out to characterize purified biomolecules. Learn the centrifugation principle, process steps, and real-life examples. Master types of centrifugation and key differences from filtration with clear diagrams. Definition of Centrifugation Centrifugation is a technique of separating substances which involves the application of centrifugal force.The particles are separated from a solution according to their size, shape, density, the viscosity of the medium and rotor speed. Sedimentation rate is directly proportional to the applied centrifugal field.