Biuret test: What is the Biuret Test

What is the Biuret Test? The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can also be used to gauge the amount of protein present in the analyte. The Biuret Test is a classic biochemical assay widely utilized to identify the presence of proteins in a given sample. This colorimetric test is based on a reaction between peptide bonds and copper ions, resulting in a distinctive color change. With its simplicity and effectiveness, the Biuret test for protein is a crucial method in biological, food, and chemical analysis laboratories. Principle of the Biuret Test The Biuret Test operates on the fundamental principle that peptide bonds in ... The biuret test is a colorimetric assay that detects the presence of at least two peptide bonds in a molecule, such as proteins. It involves a copper (II) ion forming a mauve complex with peptides in an alkaline solution, and can be modified by BCA or Lowry reagents for higher sensitivity. Principle Of Biuret Test The biuret method is a colorimetric technique specific for proteins and peptides. Copper salts in alkaline solution form a purple complex with substances containing two or more peptide bonds. In other words, Biuret test is based on the reaction of the cupric ions Cu 2+ with peptide bonds in an alkaline solution.