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A spectrophotometer is a laboratory instrument that measures the amount of light absorbed by a sample and is used to determine properties such as concentration or absorption patterns of substances. Spectrophotometry is a technique to measure the reflection or transmission of light by a material as a function of wavelength. Learn about its history, applications, types, and principles from this comprehensive article. Colour Difference is Key - Ensure Accuracy with our Advanced Colorimeters. Our Colorimeters for Unquestionable Accuracy. A spectrophotometer is a scientific instrument that measures how much light a substance absorbs. The more light a substance absorbs, the higher its concentration.