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Learn what paper chromatography is, how it works, and how to perform it with this comprehensive guide. Find out the applications, advantages, limitations, and types of paper chromatography with examples and FAQs. What is Paper Chromatography ? Paper chromatography definition explains that is an inexpensive and powerful analytical technique, which requires a piece of paper or strips serving as an adsorbent in the stationary phase across which a particular solution is allowed to pass. For the separation of dissolved chemical substances and lipid samples (in particular), paper chromatography is found to be very trustable. This analytical tool employs very few quantities of material. Principle of Paper ... Paper chromatography is a technique for separating colored or colorless chemicals on a filter paper using a solvent. Learn about its components, types, history, and applications in chemistry and biology. Paper chromatography , in analytical chemistry, a technique for separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper . It is an inexpensive but powerful analytical tool that requires very small quantities of material.