Gram positive bacteria: Gram stain is the most important

Gram stain is the most important staining procedure in Microbiology. Most bacteria can be broadly classified as Gram-positive or Gram-negative. Gram-positive bacteria stain purple , whereas gram-negative bacteria stain pink. Principle of Gram Staining When the bacteria is stained with primary stain Crystal Violet and fixed by the mordant, some of the bacteria are able to retain the primary stain and some are decolorized by alcohol. The cell walls of gram positive bacteria have a thick layer of protein-sugar complexes called peptidoglycan and lipid content is low. Gram Positive Bacteria – Examples, Benefits, Types Gram-positive bacteria are a category of bacteria that appear violet or blue when stained using the Gram staining technique, a method developed by Hans Christian Gram . This staining response is due to the thick layer of peptidoglycan in their cell walls, which retains the crystal violet stain used during the process. In contrast to Gram -negative bacteria , which have a thinner peptidoglycan layer and an outer membrane, Gram-positive ... These bacteria give a positive result in the Gram stain test by appearing purple coloured when examined under a microscope, hence named, gram-positive bacteria . Actinomyces, Clostridium, Mycobacterium, Streptococci, Staphylococci, and Nocardia are a few examples of Gram-positive bacteria .