Chromatography: • Chromatography is a method of physical

• Chromatography is a method of physical separation in which components of mixture gets separated on two phases. • One of the phase is the immobile phase is called stationary phase and the other phase is the mobile fluid that flows over the stationary phase. • During the movement of the sample, a separated result is formed by the repeated desorption and sorption in the direction of the mobile phase migration. Chromatography T HE technique of chromatography is vastly used for the separation, purification and identification of compounds. According to IUPAC, chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary while the other moves in a definite direction. Learn what chromatography is, how it works, and what are the different types of chromatography techniques. Find out how chromatography is used for separation, purification, and testing of compounds in various fields. Chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase.